Anti-HIV Type 1 p24 Clone 39/6.14
Specificity It reacts with the p24 protein of the human immunodeficiency virus type 1 (HIV 1). It is cross-reactive with human immunodeficiency virus type 2 and simian immunodeficiency virus (SIV). General notes The antibody is used to detect the HIV 1 core protein in virus-infected cells as well as lysates of infected or viral cells.…
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Soft Agar Assays for Anchorage Independent Cell Growth
Neoplastic transformation occurs via a series of genetic and epigenetic alterations that yield a cell population that is capable of proliferating independently of both external and internal signals that normally restrain growth. Anchorage-independent growth is one of the hallmarks of cell transformation, which is considered the most accurate and stringent in vitro assay for detecting the…
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A Review of Lipoproteins and Lipid Metabolism
Lipoproteins are important soft Agar Assays for Anchorage Independent Cell Growth protein-lipid assemblies that are responsible for the transport of fats to different parts of the body via the bloodstream. The five major groups of lipoproteins are very-low-density lipoprotein (VLDL), low-density lipoprotein (LDL), intermediate-density lipoprotein (IDL), high-density lipoprotein (HDL), and chylomicrons. LDL and HDL are of particular interest due to their impact on…
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Using Recombinant Adenovirus for Successful Gene Delivery
Using adenovirus to deliver genes to target cells is an option for researchers who are interested in a high titer virus with high transient expression levels that infect a wide range of cell types. Although adenovirus does not integrate into the genome of the target cell the way a retrovirus can, it is an excellent choice when only transient…
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Adeno-Associated Virus (AAV) Provides Advantages for Gene Delivery
Recombinant Adeno-associated virus (AAV) is often the preferred method for delivering genes to target cells due to its high titer, mild immune response, ability to infect a broad range of cells, and overall safety. Native AAV can infect humans and primates; however, it has not been reported to cause disease and poses minimal risk to humans. Compared with…
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Methods for Measuring Cell Proliferation
Cell proliferation is an increase in the number of cells resulting from the normal, healthy process by which cells grow and divide. In this regard, cell proliferation can be a good indicator of general cell health. Cells that are subject to a variety of disease states may exhibit different rates of proliferation than normal cells. Therefore,…
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Measuring Cellular Hypoxia
Hypoxia, or low oxygen condition, is a normal physiological response to certain body stressors such as high altitudes. Such a condition can result in dizziness, light-headedness, nausea, and related reactions. These reactions may be a result of hypoxic conditions at the system level or the cellular level or both. Cellular hypoxia can also be a…
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