Using Recombinant Adenovirus for Successful Gene Delivery
Using adenovirus to deliver genes to target cells is an option for researchers who are interested in a high titer virus with high transient expression levels that infect a wide range of cell types. Although adenovirus does not integrate into the genome of the target cell the way a retrovirus can, it is an excellent choice when only transient…
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Adeno-Associated Virus (AAV) Provides Advantages for Gene Delivery
Recombinant Adeno-associated virus (AAV) is often the preferred method for delivering genes to target cells due to its high titer, mild immune response, ability to infect a broad range of cells, and overall safety. Native AAV can infect humans and primates; however, it has not been reported to cause disease and poses minimal risk to humans. Compared with…
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Methods for Measuring Cell Proliferation
Cell proliferation is an increase in the number of cells resulting from the normal, healthy process by which cells grow and divide. In this regard, cell proliferation can be a good indicator of general cell health. Cells that are subject to a variety of disease states may exhibit different rates of proliferation than normal cells. Therefore,…
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Measuring Cellular Hypoxia
Hypoxia, or low oxygen condition, is a normal physiological response to certain body stressors such as high altitudes. Such a condition can result in dizziness, light-headedness, nausea, and related reactions. These reactions may be a result of hypoxic conditions at the system level or the cellular level or both. Cellular hypoxia can also be a…
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