• May 29, 2021

Recombinant Human Ephrin Type-B

What does CF mean?

CF stands for Carrier Free (CF). We generally add bovine serum albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein improves the stability of the protein, increases shelf life, and allows the recombinant protein to be stored in a more dilute concentration. The free version of the operator does not contain BSA.

Which formulation is right for me?

In general, we recommend purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, carrier-free protein is recommended for applications where the presence of BSA could interfere.

Formulation: Lyophilized formulation from a 0.2 µm filtered solution in PBS.

Reconstitution: Reconstitute at 200 μg / mL in sterile PBS.

Shipping: The product is shipped at room temperature. Upon receipt, please store immediately at the recommended temperature below.

Stability and Storage:

  • Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 ° C as supplied.
  • 1 month at 2 to 8 ° C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 ° C under sterile conditions after reconstitution

Background: EphB2

EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Dirt, is a 125 kDa member of the transmembrane Eph receptor tyrosine kinase family that binds members of the Ephrin family on adjacent cells. The interaction triggers forward signaling in the receptor-expressing cells through the Eph receptor and reverses signaling in the ligand-expressing cells through Ephrin. Human EphB2 cDNA encodes a 1055 amino acid (aa) precursor, which includes an 18 aa signal sequence, a 525 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 491 aa cytoplasmic domain.

The ECD contains a cysteine-rich region followed by two fibronectin type III domains. The cytoplasmic domain contains the tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif. Human EphB2 shares 99% aa sequence identity with mouse and rat EphB2 within the ECD region. A short isoform that lacks 70 aa at the C-terminus has also been reported.

Hippocampal neurons can release vesicles containing full-length EphB2, and these are taken up by neighboring glial cells. EphB2 is expressed on both sides of the neuronal synapse. It controls axon guidance across the embryonic midline, promotes a neuronal fate from neural precursors, and regulates NMDA receptor activity. EphB2 interaction with Ephrin-A5 promotes axonal growth cone collapse, while its interaction with Ephrin-B ligands is required for the inner ear, renal, anorectal, and vascular development.

Signaling in Ephrin expressing cells through the EphB2-Ephrin complex requires proteolytic cleavage of EphB2 that releases its extracellular domain. Following the shedding of the extracellular domain of EphB2, the cytoplasmic domain of EphB2 is released from the plasma membrane by the presenilin-dependent gamma-secretase activity to initiate a signaling cascade in the EphB2 expressing cells. Aberrant EphB2 expression and activity are implicated in the progression of several cancers.

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